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Figure 5
Substrate binding shown in two views. Polar contacts are shown as magenta dashed lines. A salt bridge between Lys587 and the substrate with a distance of longer than 3.5 Å is shown as a cyan dashed line. Only waters connecting the ligand and protein are shown. (a, b) Tryptophan (orange C atoms) bound to AetF (yellow C atoms) from PDB entry 8cje chain C. (c, d) 5-Br-Trp (dark green C atoms) bound to AetF (green C atoms) from PDB entry 8cjf chain A. Schematic representations of the indole moieties of tryptophan and 5-bromotryptophan with numbered C atoms are added. The grey dashed lines indicate the rotational axis between the binding poses of both ligands.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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