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Figure 4
Example of two ZART reconstructions representing the two main conformational states in the EMPIAR-10028 data set. The two states correspond to the conformations present in the data set, corresponding to a movement of the small subunit of the ribosome. Thanks to ZART, it is possible to reconstruct these two states without sacrificing any particles, thus increasing the resolution of the resulting cryoEM maps. (a) Comparison of the two states reconstructed by ZART. The comparison shows the motion of the small subunit of the ribosome. Arrows are added to aid the visualization of the conformational change. (b) Visualization of the two reconstructed maps. As expected, both maps show similar features, even though one of the reconstructed states has a much lower representation in the landscape.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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