Figure 1
Cartoon representation of the TtLMCO1 crystal structure. Each domain (A, B and C) is coloured differently: in cyan, raspberry and green, respectively. The disulfide bond between Cys28 and Cys236 is shown in stick representation. Copper ions and a dioxygen molecule located at the TNC are shown as brown and red spheres, respectively. Glycans are depicted as cyan sticks. (a) A NAG molecule is modelled into a 2Fo − Fc electron-density map contoured at 1σ. A hydrogen bond is formed by the main-chain carbonyl O atom of Ile16 and the N2 atom of NAG (yellow dotted line). (b) Two NAG molecules are modelled into a 2Fo − Fc electron-density map contoured at 1σ. A hydrogen bond is formed between the OD1 atom of Asp420 and the N2 atom of NAG (yellow dotted line). A CH–π interaction is formed between the phenyl group of Tyr595 and the D-glycopyranose ring of NAG (red dotted line). |