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Figure 3
Crystal structure of an isolated helix 7 from the N-terminal domain (NTD) of H. pylori DnaB helicase (HpDnaB). Comparison of deposited (a) and corrected (b) models. The peaks in the difference-density mFo − DFc map can be eliminated by shifting the model sequence register by two residues. For example, the clearly too large Phe C/111 resulting in a prominent negative (red) difference-density peak is replaced with a smaller Leu C/113 in the model with the corrected sequence register. Similarly, a clear positive peak (green) near Asn C/115 is interpreted as an Ile C/117 side chain in the corrected model. The combined 2mFoDFc (blue) and difference mFoDFc (red/green) maximum-likelihood maps calculated using REFMAC5 and PDB_REDO are shown at 2σ and 3σ levels, respectively. At this threshold no difference-density map features are visible in the presented asymmetric unit fragment of the corrected model.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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