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Figure 7
Effect of DMF on the affinity of the three proteins for iron(III)-azotochelin. The binding curves for CjCeuE (a), Gst (b) and Pth (c) were obtained by intrinsic fluorescence quenching in the absence of (black squares) and the presence of 10% (blue triangles) and 20% (red circles) DMF. The Kd values were estimated from the fitted curves using equation (1)[link] and expressed relative to their respective 0% DMF control (d). The buffer was 40 mM Tris–HCl pH 7.5, 150 mM NaCl. Curves were collected in duplicate and averaged. Data with respective mean absolute deviations are available in Supplementary Fig. S7.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 79| Part 8| August 2023| Pages 694-705
https://doi.org/10.1107/S2059798323004473
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