Module walking using an SH3-like cell-wall-binding domain leads to a new GH184 family of muramidases

Moroz, O.V.; Blagova, E.; Lebedev, A.A.; Skov, L.K.; Pache, R.A.; Schnorr, K.M.; Kiemer, L.; Friis, E.P.; Nymand-Grarup, S.; Ming, L.; Ye, L.; Klausen, M.; Cohn, M.T.; Schmidt, E.G.W.; Davies, G.J.; Wilson, K.S.

doi:10.1107/S2059798323005004

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 1
SDS–PAGE analysis of TsCWBD in the supernatant after incubation with Avicel (control) or M. lysodiekticus cells shows a reduction in the TsCWBD content in the supernatant after incubation with M. lysodiekticus cells. Lane 1, molecular-weight marker (from the bottom: 10, 15, 25, 35, 55, 70, 100, 130 and 250 kDa); lane 2, TsCWBD incubated with Avicel; lane 3, TsCWBD incubated with M. lysodiekticus cells; lane 4, untreated TsCWBD.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 8| August 2023| Pages 706-720

https://doi.org/10.1107/S2059798323005004

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