journal menu
![[Figure 1]](vo5014fig1mag.jpg)
|
|
Figure 1
The Raynals pipeline has four steps. Firstly, the second-order autocorrelation data are loaded and filtered based on initial value and baseline quality. Input parameters include the detection angle, laser wavelength, temperature, refractive index and viscosity. Secondly, a regularization approach is used to fit the first-order autocorrelation data, assuming a smooth nonparametric distribution of decay rates (or hydrodynamic radii). Thirdly, a threshold based on residuals can be used to remove poorly fitted curves and the estimated distributions are displayed. The user must select regions of interest to extract information about the peaks (for example the contribution to the total intensity). Finally, the user can export the Rh distribution and the associated second-order autocorrelation curves. |
![[Figure 1]](vo5014fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
access
