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![[Figure 1]](di5066fig1mag.jpg)
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Figure 1
1TEL-AA-vWa (SUMO) crystal lattice. (a) Schematic of a 1TEL fusion indicating the variables investigated in this study. (b) Batch 2 1TEL-AA-vWa (SUMO) crystal fan. The scale bar is 100 µm. (c) Crystal lattice packing of the 1TEL-AA-vWa (SUMO) crystal, indicating the areas highlighted in subsequent panels. The TELSAM domains of chains A, B and C are shown in cyan, green and magenta, respectively, while the vWa domains of chains A and B are shown in yellow and brown, respectively. The unit cell is delineated by a thin black line. (d) Electron density (slate mesh) for the chain B vWa domain, contoured at 1.0σ. The 1TEL (green) and vWa (brown) domains are shown in cartoon representation for reference. (e) Detail of the region where the chain C vWa domain is expected to be found. A modelled orientation of the chain C vWa domain (white) is superimposed over the UNX atoms (blue spheres) used to delineate the unassigned electron density in this region. (f) Detail of the linker between the chain A 1TEL (cyan) and vWa domains (yellow). The electron density (slate mesh) is contoured at 1.0σ. (g) Detail of the interaction between the chain A vWa domain (yellow) and its host 1TEL domain (cyan). The proteins are shown in cartoon representation with selected amino acids shown as sticks and transparent spheres. (h) As in (g) but showing the interactions between the chain A vWa domain (yellow) and the chain B 1TEL domain (green). (i) As in (g) but showing the interactions between the chain A vWa domain (yellow) and two chain B vWa domains (brown). (j) Superposition of chain A and chain B 1TEL-AA-vWa chains via their 1TEL domains. (k) As in (g) but showing the chain B vWa (brown) and its host 1TEL (green). |
![[Figure 1]](di5066fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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