Increasing the bulk of the 1TEL–target linker and retaining the 10×His tag in a 1TEL–CMG2-vWa construct improves crystal order and diffraction limits

Gajjar, P.L.; Pedroza Romo, M.J.; Litchfield, C.M.; Callahan, M.; Redd, N.; Nawarathnage, S.; Soleimani, S.; Averett, J.; Wilson, E.; Lewis, A.; Stewart, C.; Tseng, Y.-J.; Doukov, T.; Lebedev, A.; Moody, J.D.

doi:10.1107/S2059798323007246

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 2
The results of adding bulk to the 1TEL–vWa linker. (a) Linker detail of the 1TEL-AA-vWa (SUMO) structure (PDB entry 7n1o) with 1TEL in magenta, the Ala-Ala linker in yellow and vWa in cyan. The linker and surrounding amino acids are shown as sticks and transparent spheres. (b) As in (a) but for the design model of 1TEL-AV-vWa (SUMO). (c) As in (a) but for the design model of 1TEL-TV-vWa (SUMO). (d) As in (a) but for the design model of 1TEL-TT-vWa (SUMO). (e) Representative crystals of 1TEL-TV-vWa (SUMO). The scale bar is 100 µm. (f) As in (e) but for 1TEL-AV-vWa (SUMO). (g) Representative diffraction pattern of a 1TEL-TV-vWa (SUMO) crystal. (h) As in (g) but for a 1TEL-AV-vWa (SUMO) crystal.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 10| October 2023| Pages 925-943

https://doi.org/10.1107/S2059798323007246

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