Increasing the bulk of the 1TEL–target linker and retaining the 10×His tag in a 1TEL–CMG2-vWa construct improves crystal order and diffraction limits

Gajjar, P.L.; Pedroza Romo, M.J.; Litchfield, C.M.; Callahan, M.; Redd, N.; Nawarathnage, S.; Soleimani, S.; Averett, J.; Wilson, E.; Lewis, A.; Stewart, C.; Tseng, Y.-J.; Doukov, T.; Lebedev, A.; Moody, J.D.

doi:10.1107/S2059798323007246

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 4
Molecular details of 1TEL-TV-vWa (SUMO). (a) Schematic of the unit cell, indicating the areas highlighted in subsequent panels. (b) Detail of the interaction between the chain A vWa domain (yellow) and its host 1TEL domain (cyan). The protein is shown in cartoon representation with selected side chains shown as sticks and transparent spheres. (c) As in (b) but for the interaction between the chain A vWa and a 1TEL domain from the next turn of the host polymer. (d) As in (b) but for the interaction between the chain A vWa domain (yellow) and two chain B vWa domains (brown). (e) As in (b) but for the interaction between the chain C vWa domain (light blue) and an adjacent, symmetry-related chain C vWa domain (dark blue). (f, g) Comparison of the antiparallel (f) polymer–polymer interaction between chain A (cyan and yellow) and chain B (green and brown) and the parallel (g) polymer–polymer interaction between two copies of chain C (magenta and dark blue).

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 10| October 2023| Pages 925-943

https://doi.org/10.1107/S2059798323007246

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