Increasing the bulk of the 1TEL–target linker and retaining the 10×His tag in a 1TEL–CMG2-vWa construct improves crystal order and diffraction limits

Gajjar, P.L.; Pedroza Romo, M.J.; Litchfield, C.M.; Callahan, M.; Redd, N.; Nawarathnage, S.; Soleimani, S.; Averett, J.; Wilson, E.; Lewis, A.; Stewart, C.; Tseng, Y.-J.; Doukov, T.; Lebedev, A.; Moody, J.D.

doi:10.1107/S2059798323007246

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 6
Crystallization and structural details of His-1TEL-TV-vWa. (a) Representative crystals of 1TEL-TV-vWa. The scale bar is 100 µm. (b) Representative diffraction pattern from a His-1TEL-TV-vWa crystal. (c) Unit-cell packing of a His-1TEL-TV-vWa crystal with the 1TEL domain in magenta and the vWa domain in slate. The unit cell is shown as a thin black line. (d) Superposition of the structure of His-1TEL-AA-vWa (magenta and wheat) with the structure of His-1TEL-TV-vWa (magenta and slate) on their 1TEL domains. (e) Detail of the interaction between the vWa domain (slate) and its host 1TEL domain (magenta). The protein is shown in cartoon representation with selected side chains shown as sticks and transparent spheres. (f) As in (e) but showing the interaction between the vWa domain (slate) and a vWa domain (blue) from an adjacent polymer.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 79| Part 10| October 2023| Pages 925-943

https://doi.org/10.1107/S2059798323007246

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