Figure 10
B-factor analysis of AaHypD-C360S structures obtained from different clusters in intensity-based HCA. (a) Plot of the B factors of Cα atoms in data from different clusters. (b, c) Main-chain trace of AaHypD-C360S with an `unfolded' N-terminus obtained from Cluster 50 (b) and with a `folded' N-terminus obtained from Cluster 52 (c). B-factor-based coloring was applied using Coot. The N-terminus and C-terminus are depicted in the figure as `N' and `C', respectively. The structure with the `unfolded' N-terminus showed a significantly high B factor around the [4Fe–4S] cluster. The figures were generated by UCSF Chimera (Pettersen et al., 2004). |