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![[Figure 2]](jc5061fig2mag.jpg)
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Figure 2
Domain prediction and phylogenetic analysis of Mef1. (a) Domain predictions of selected fucoidanases: Mef1, P5AFcnA/P19DFcnA, MfFcnA and Mef2. Yellow, signal peptide; blue, D1 (β/α)8 GH107 catalytic domain; pink, Ig-like domain (IPR003343); green, secretion-system C-terminal sorting domain (T9SS domain, T9; IPR026444); red, galactose binding-like domain (IPR000421); gray, FA58C domain (IPR000421) and FTP1 domain (IPR006585); orange, CBM6 domain (IPR005084); purple, invasin/intimin cell-adhesion domains (IPR008964). Domains were predicted using SignalP (signal peptide), InterProScan and sequence alignment (D1) with P5AFcnA and MfFcnA. (b) Phylogenetic analysis of the D1 domain amino-acid sequences of selected GH107 fucoidanases to help display how Mef1 is separated from other GH107 enzymes (enzyme codes and the microbial origin of each enzyme are given in Section 2.3 ![[link]](../../../../../../logos/arrows/d_arr.gif){kind=small} ); the numbers after each enzyme code denote the sequence frame used in the analysis for each protein. The phylogenetic tree was constructed with maximum likelihood using phyML and supported by likelihood-ratio tests resulting in values from 0 to 1, comparable to bootstrap values. The scale bar at the bottom and the number 0.07 is an indicator of the genetic distance based on branch length. Turquoise, α(1,3)-linkage-specific fucoidanases; green, Mef1; blue, α(1,4)-linkage-specific fucoidanases. |
![[Figure 2]](jc5061fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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