view article
Figure 4
Substrate specificity of Mef1 on different brown seaweed fucoidans by carbohydrate polyacrylamide gel electrophoresis (C-PAGE) after 2 h of reaction. Lanes C, fucoidan substrate controls for each type of fucoidan; lanes E, enzyme action of Mef1 on the fucoidans from F. evanescens (Fe), S. mcclurei (Sm), T. ornata (To), S. polycystum (Sp), H. cuneiformis (Hc), S. latissima (Sl), S. oligocystum (So), S. serratum (Ss) and F. vesiculosus (Fv). St designates the hydrolysate standard obtained after the enzymatic reaction of FFA2 [an α(1,4)-linkage-cleaving GH107 fucoidanase] on F. evanescens fucoidan with all fucose residues sulfated at C2; the lowest band is a tetrasaccharide (Silchenko et al., 2017BB31).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 79| Part 11| November 2023| Pages 1026-1043
https://doi.org/10.1107/S2059798323008732
Follow Acta Cryst. D
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS