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![[Figure 1]](nj5320fig1mag.jpg)
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Figure 1
Disulfide-bridge formation as a strategy to capture disulfide-linked DUB–Ub structures. (a) A list of the DUBs tested for disulfide reaction. Eukaryotic and prokaryotic DUBs are grouped according to their families. (b) A schematic of UbG76C (yellow) disulfide-bridge formation with a target DUB (orange) utilizing DTNB. (c) SDS–PAGE analysis under nonreducing conditions of the reaction mixtures of DUBs with the UbG76C–TNB adduct. The red boxes indicate examples of DUBs reacting with the Ub reagent. |
![[Figure 1]](nj5320fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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