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![[Figure 2]](nj5323fig2mag.jpg)
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Figure 2
PTE variants. (a) Schematic presentation of the maltose-binding protein (MBP) fused before the factor Xa cleavage motif (IEGR) and the octapeptide spacer sequence, 26ISEFITNS33, followed by the mature PTE protein sequence starting with Gly34. Factor Xa cleaves after the arginine residue of the cleavage motif, leaving the 26ISEFITNS33 linker attached to the PTE, Gly34–Ser365. (b) Sequence alignment of wt PTE, PDB entry 1hzy, A53, C23, C23M, A53_T, C23_T and C23M_T. The last three bear the octapeptide tag. Secondary-structure elements of PDB entry 1hzy are labeled above the alignments: α-helices and 310-helices (shown with the symbol η) are indicated by coils and β-strands by arrows. Residues conserved in all variants are in red. Multiple sequence alignment was performed using MultAlin (Corpet, 1988  ) and the figure was created using ESPript (Robert & Gouet, 2014). |
![[Figure 2]](nj5323fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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