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![[Figure 1]](qe5005fig1mag.jpg)
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Figure 1
Map examples. (a) Electron density maps for Tyr12 (PDB entry 3kyu, 1.1 Å resolution) show positive peaks for all aromatic H atoms; however, the H atom on the hydroxyl group is not visible. (b) Cryo-EM maps for Tyr32 (PDB entry 7a4m, 1.22 Å resolution) show positive difference peaks for all H atoms. (c) Neutron scattering length density maps for Tyr146 (PDB entry 1cq2, 2.0 Å resolution) show positive difference peaks for all H atoms (in the form of 2H), including the H atom on the hydroxyl group. Electron and neutron scattering length density maps were calculated using REFMAC5 (Murshudov et al., 2011  ), contoured at the +1.0σ (2mFo − DFc in grey) and +3.0σ (mFo− DFc in green) levels. Cryo-EM weighted and sharpened Fo (grey) and omit (Fo − Fc, green) maps were calculated using Servalcat (Yamashita et al., 2021) and contoured at the +1.5σ and +3.0σ levels, respectively. Molecular-graphics representations were produced with Coot 1.0 (Emsley et al., 2010). |
![[Figure 1]](qe5005fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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