Figure 2
Absorbance spectra of CATPO and its E484A, E484I, E484D, T188A, T188D, T188F and T188I variants in 0.1 M sodium phosphate buffer pH 7.0 at room temperature. The wild-type CATPO spectrum is fitted so that the Soret peak for each of the variants has an equivalent value. Full spectra are provided in Supplementary Fig. S10. |