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![[Figure 3]](ic5122fig3mag.jpg)
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Figure 3
Representative use cases of the `Analyse Micrographs' and `Analyse Particles' tabs in relion_analyse.py. (a) After pre-processing (motion correction and CTF estimation) of a data set, a common approach is to manually select thresholds to discard low-quality micrographs. The red dotted lines represent sensible thresholds for CtfMaxResolution and CtfFigureOfMerit. Colouring the plot by ice score (calculated by ice.py) shows a group of micrographs with low-quality ice. These would be included for further processing with the manual thresholds but can be easily removed using the lasso tool. (b) Angular distribution of particles (AngleTilt versus AngleRot) resulting from merging data sets collected at 0° and 40° tilt, respectively, and plotted in the `Analyse Particles' tab. Colouring by OpticsGroup, we can observe that the tilted data set (optics group 1, purple) fills up the orientational space, providing views that are absent in the 0° data set (optics group 2, yellow). Data were obtained from EMPIAR-10096 and EMPIAR-10097 (Tan et al., 2017  ). |
![[Figure 3]](ic5122fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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