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![[Figure 4]](qi5004fig4mag.jpg)
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Figure 4
High-pressure cooling process. (a) The macromolecular crystal is harvested from a crystallization tray and loaded into the pressure tube at ambient pressure and temperature. The pin with the crystal is held in the upper part of the pressure tube by a strong magnet (M). (b) The crystal is pressurized in the tube at ambient temperature. (c) The crystal is cryo-cooled in the tube under pressure by releasing it from the upper part and dropping it abruptly into the lower part of the tube, which is maintained at a regulated cryogenic temperature. (d) The crystal is recovered manually from the tube at ambient pressure within the cryogenic bath. The red arrows indicate the three steps of the high-pressure cooling thermodynamic pathway (from Fig. 2 ![[link]](../../../../../../logos/arrows/d_arr.gif){kind=small} ). |
![[Figure 4]](qi5004fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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