Figure 6
Access to the active site in the haloalkane dehalogenase DhaA. (a) Structure of native DhaA; the active site is connected to the solvent by a main tunnel and a slot tunnel (PDB entry 4hgz). (b) Structure of the krypton derivative of the mutant DhaA115 (PDB entry 6sp8). Although the accesses to the active site of DhaA115 are closed at atmospheric pressure (Caver calculations), the krypton derivative (pressurized at 140 bar) reveals a series of Kr atoms demonstrating the effective accessibility of the active site. |