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![[Figure 1]](qu5005fig1mag.jpg)
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Figure 1
Overview of common strategies that can be used to capture the structure of reaction intermediates using light as the reaction trigger. Protocols implicating cryogenic temperature data collection are represented with a blue background: to block a reaction initiated at room temperature once an equilibrium is reached (`frozen equilibrium'), or before it is reached (`trigger–freeze') or to limit the progress of a reaction by initiating it at cryogenic temperature (`freeze–trigger'). Protocols implicating room-temperature data collection are represented on a red background: to maintain a reaction at equilibrium (`steady-state equilibrium') or to catch intermediates as the reaction proceeds (`pump–probe'). For simplicity, only protocols relying on photoactivation are depicted here. Meaning of symbols: cyan rectangles, UV–visible actinic light; yellow rays, X-rays; red waves, room temperature; blue snowflake, cryogenic temperature |
![[Figure 1]](qu5005fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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