Figure 7
Diffusion-based time-resolved experiments. (a) Strategies enabling the initiation of a reaction by the diffusion of a substrate or a cofactor. (b) Overview of studies conducted on emblematic targets: fluoroacetate dehalogenase (depicted using PDB entry 5k3a; Mehrabi, Schulz, Dsouza et al., 2019), β-lactamase (depicted using PDB entry 7bh5; Butryn et al., 2021) and xylose isomerase (depicted using PDB entry 8aw8; Mehrabi et al., 2023). Colour code: protein secondary structure, white; substrate, orange; ions, green (chloride ion), red [magnesium(II) ion] and lilac [manganese(II) ion]. |