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![[Figure 4]](ih5004fig4mag.jpg)
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Figure 4
The assembly pathway of complex I in Y. lipolytica. (a) The central subunit ND2 and accessory subunit NDUFC2 associate with the assembly factors NDUFAF1 and CIA84 and with tafazzin to form the early PP module intermediate. (b) The central membrane subunits ND4L, ND3, ND6 and ND1 are added successively, whereby the TMH1–2 loop of ND3 is secured by NDUFAF1. The addition of the accessory subunits NDUFA1, NDUFA3, NDUFA8, NDUFA13 (one TMH each), NUXM (two TMHs) and NDUFS5 (IMS side) completes the late PP module intermediate. (c) The C-terminal domain of NDUFB5 on the IMS side makes a large contribution to joining the PD and PP modules. The core of the Q module is anchored by the N-terminal coil of NDUFA13. The assembly factors are released and ND3, ND6 and ND1 rearrange to accommodate the Q-module interaction partners. (d) The assembly factor NDUFAF2 attaches to the Q module. (e) The N module binds to the Q module, interacting with the C-terminal part of NDUFAF2. (f) The combined action of NDUFS4, NDUFS6 and NDUFA12 releases the assembly factor, leading to mature complex I. |
![[Figure 4]](ih5004fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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