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Figure 2
Bragg intensity scaling results from a multi-crystal insulin data set. Rotation data sets of 50° (500 frames each) were collected from 17 locations on two large insulin crystals (pictured). Each 50° wedge (sweep) spans the width of the white/gray vertical bars. Correction factors from global scaling in DIALS are shown for each wedge. The illuminated volume changes during rotation, as reflected in the overall scale factor (top axes, blue curves). B factors show a characteristic linear decay with exposure time due to radiation damage (bottom axes, orange curves). The overall extent of radiation damage is consistent among the data sets.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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