Figure 3
Simulated template matching of thermosomes. (a) Slice through a simulated tomogram containing randomly placed and oriented thermosomes (PDB entry 3j1b). (b) Score map from matching with a single orientation. The peak boxed in magenta shows an ideal match with a strong CCC peak; the peak boxed in blue does not match well and has a correspondingly small peak. (c) Final score map after full orientational search. (d) Score map from (c) thresholded to remove background so that only true peaks remain. The color map shows the normalized CCC score, where 1 denotes the maximum value in the map and 0 denotes the lowest value in the map. |