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Figure 4
Comparison of template matching in STOPGAP with different filters. (a) Slice through a tomogram taken from S. cerevisiae lamella (EMPIAR-11658). Ribosome template matching with a 15° angular step with (b) no CTF or exposure filter, (c) CTF and exposure filtering and (d) CTF and exposure filtering and noise correlation. Blue boxes indicate CCC scores around a membrane bilayer, while magenta boxes indicate scores around a true positive. The color map shows normalized CCC score, where 1 denotes the value at the CCC peak and 0 the lowest value in the map; the score maps in (b), (c) and (d) have not been thresholded.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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