Figure 7
Comparison of HIV s-CANC structures determined using different missing-wedge models. (a) Fourier shell correlation (FSC) plots of HIV s-CANC structures determined from the five-tomogram subset of EMPIAR-10164 by AV3 (Turoňová et al., 2017) using a solid wedge mask and STOPGAP using CTF and exposure filtering. Both used novaCTF (Turoňová et al., 2017) for 3D CTF-corrected tomogram reconstruction with no tilt-series refinement. (b) and (c) are example isosurface representations of the AV3 and STOPGAP EM density maps, respectively. An atomic model (EMD-3782) is rigid-body fitted into both maps. (d–g) XY and XZ orthographic slices through the EM density maps determined by AV3 and STOPGAP, respectively. The red arrow indicates the high negative density region in the AV3 map that is not present in the STOPGAP map. |