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Figure 2
Schematic illustrating the n-helix tilt scheme for n = 2 overlapping tilt series with 50% overlap between sweeps. (a) The overlapping data-collection areas as the beam is shifted along the axis of the pillar sample. (b) The order of image acquisition between −90° and −76° is illustrated by image number and colour as light (first image) to dark (last image). The beam is shifted along the pillar to acquire tilt images at each angle before tilting the stage to the next angle. In this example, the beam is then shifted again along the sample to overlap the previous acquisition area by 50% and the intermediate angles which were skipped in the first pass are then acquired at each beam-shift position.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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