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![[Figure 1]](rr5238fig1mag.jpg)
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Figure 1
Examples of anisotropic cryo-EM maps resulting from orientation bias. The upper panel shows the reference-free 2D class averages of (a) SARS-CoV-2 spike protein and (b) human erythrocyte catalase. For the spike protein, preferred bottom views are observed. In the case of catalase, a preference for the top/bottom view is evident. In the lower panel, 3D maps with anisotropic features are shown for the preferred and perpendicular views as labelled. The symmetries applied during reconstruction were C1 and D2 for the spike protein and catalase, respectively. |
![[Figure 1]](rr5238fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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