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![[Figure 2]](rr5238fig2mag.jpg)
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Figure 2
Representative micrographs, with a few selected particles indicated with red circles, and 2D class averages of the test proteins used in this study. (a) The C-reactive protein (CRP) pentamer adopts a preferred bottom view, which shows the pentameric arrangement of the monomers. (b) The CRP decamer adopts a preferred side view, which shows the staggered arrangement of two CRP pentamers stacked on top of each other. The same micrograph is used in (a) and (b). (c) Catalase adopts a preferred top view, as seen in the micrograph and 2D class averages. (d) SARS-CoV-2 spike adopts a preferred bottom view showing the trimeric arrangement. (e) PaaZ adopts a preferred side view, as seen in the 2D class averages, and the micrograph shows occasional clumping of hexamers on the grids. (f) β-Galactosidase with an N-terminal polyhistidine tag adopts a preferred side view, as seen in the 2D class averages, and the micrograph shows aggregation on grids. For the above data sets, the catalase and PaaZ grids were prepared at 4°C and all other grids were prepared at 20°C. |
![[Figure 2]](rr5238fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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