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![[Figure 5]](rr5238fig5mag.jpg)
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Figure 5
The effect of temperature during cryo-EM sample preparation of catalase and PaaZ. Micrographs, maps, orientation-distribution plots and the different parameters that are used to analyse the quality of the maps are shown. N indicates the number of particles used for reconstruction, R indicates the final resolution of the map, S indicates the sphericity and Eod indicates the efficiency of Fourier space coverage. (a) Catalase orientation distributions change significantly when grids are blotted at different temperatures in the absence of any additive. (b) PaaZ orientation distributions change slightly when grids are held and blotted at different temperatures in the absence of any additive. In the case of PaaZ, the condition with grids prepared at 4°C with CTAB as an additive is included for comparison as this combination led to a high-resolution isotropic map. |
![[Figure 5]](rr5238fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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