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Figure 1
The Cryo2RT workflow. Samples are mounted on IMISX chips or cryoloops, snap-cooled and stored in liquid nitrogen (LN2) before shipping to the synchrotron. At the beamline, the samples are mounted on the goniometer using a sample changer under cryo conditions. RT data are collected after stopping the LN2 cryostream (RT2) after the first data set (cryo1) has been collected under cryo conditions at 100 K. Finally, the sample can be cryo-cooled again for another cryo data collection (cryo3) and stored back in LN2.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 80| Part 8| August 2024| Pages 620-628
https://doi.org/10.1107/S2059798324006697
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