Microcrystal electron diffraction structure of Toll-like receptor 2 TIR-domain-nucleated MyD88 TIR-domain higher-order assembly

Li, Y.; Pacoste, L.C.; Gu, W.; Thygesen, S.J.; Stacey, K.J.; Ve, T.; Kobe, B.; Xu, H.; Nanson, J.D.

doi:10.1107/S2059798324008210

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 2
Incubation of TLR1^TIR or TLR6^TIR with MyD88^TIR does not induce higher-order assembly formation. No significant increase in turbidity was observed and negative-stain EM did not show the presence of higher-order assemblies. (a) Turbidity-based polymerization assays testing the effect of TLR1^TIR on higher-order assembly formation. (b) Negative-stain EM analysis of 30 µM TLR1^TIR mixed with 60 µM MyD88^TIR. (c) Turbidity-based polymerization assays testing the effect of TLR6^TIR on MyD88^TIR higher-order assembly formation. (d) Negative-stain EM of 30 µM TLR6^TIR mixed with 60 µM MyD88^TIR. Assays were repeated at least twice with similar results.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 80| Part 9| September 2024| Pages 699-712

https://doi.org/10.1107/S2059798324008210

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