Microcrystal electron diffraction structure of Toll-like receptor 2 TIR-domain-nucleated MyD88 TIR-domain higher-order assembly

Li, Y.; Pacoste, L.C.; Gu, W.; Thygesen, S.J.; Stacey, K.J.; Ve, T.; Kobe, B.; Xu, H.; Nanson, J.D.

doi:10.1107/S2059798324008210

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 6
MicroED data collection of TLR2^TIR-induced MyD88^TIR microcrystals. (a) Illustration of a sample grid employed for data acquisition. (b) An individual MyD88^TIR microcrystal resting on the grid. (c) Diffraction pattern derived from a single TLR2^TIR-induced MyD88^TIR microcrystal after exposure to a fluence of ∼0.14 e Å⁻² (0° rotation), showing visual spots up to 2.60 Å resolution, as highlighted in the figure. (d) Diffraction pattern towards the end of data collection (∼4.4 e Å⁻²; 32.5° rotation), with a highlighted reflection visible at 3.05 Å resolution. The ring illustrates the data resolution cutoff for processing and final refinement.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 80| Part 9| September 2024| Pages 699-712

https://doi.org/10.1107/S2059798324008210

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