Figure 3
Crystal contacts and packing of NowGFP molecules in the orthorhombic crystal structure. The secondary structures of NowGFP are represented as ribbons, and atoms belonging to crystal contacts are represented as surfaces. (a) Contact IA is viewed along the c axis. (b) Contacts IB and VB are viewed along the c axis. (c) Contacts IA and IB are viewed along the b axis. (d) Contacts IIAB, IIIAB and IVAB are viewed along the a axis. Contacts IA, IB, IIAB, IIIAB, IVAB and VB are coloured blue, yellow, red, green, light blue and grey, respectively. (e) Schematic diagram of protein packing. Crystal contacts are represented by coloured arrows and linear assemblies connected by contact IA are represented as blue- or purple-coloured boxes, while linear assemblies connected by contact IB are represented as yellow- or orange-coloured boxes. The linear assemblies connected by contacts IA and IB are linked along the a axis and the b axis, respectively. Linear assemblies composed of chains A and B are stacked perpendicular by contacts IIAB, IIIAB and IVAB along the c axis. Linear assemblies composed of chain B are stacked parallel by contact VB along the a axis. The symmetry operation between two assemblies composed of the same chains, 1A–2A and 3A–4A (or 1B–3B and 2B–4B), indicated by opposite white arrows, is (−x + 1/2, −y, z + 1/2). |