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![[Figure 4]](qu5006fig4mag.jpg)
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Figure 4
Solution of target structure PDB entry 8bfi (3 Å resolution, one copy in the asymmetric unit, space group P21) using PAE-based domain splitting of ColabFold predictions of each of the three chains to create suitable search models for MR. The target structure is shown in the centre, coloured by its three chains: A (blue), B (gold) and C (orange). Chains A and C have domain-swapped parts, leading to large differences in the relative orientation of each of the domains of the predicted chain when compared with the crystallized form. However, utilizing the PAE matrices produced by ColabFold (shown for each chain; blue is low error and red is high error), the domains can be separated out from the chain predictions to produce accurate search models (shown in the boxes) that can be placed correctly in MR by Phaser. All predicted domains were placed correctly apart from A1, which was not present in the crystal, and A3 and B2, which both constituted a very small fraction of the overall scattering content. The TFZ from Phaser for each search-model placement is shown (values above 8 are indicative of correct placement). The final LLG for all of the placed components was 2048. |
![[Figure 4]](qu5006fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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