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Figure 2
Equilibrium unfolding of LtPHBase in GdHCl. The black circles represent the average percentage of unfolded protein determined by three independent experiments. The line is a fit of the data to a two-state unfolding mechanism. The left inset shows the fluorescence emission intensity spectrum for the concentration points measured (0.0 M GdHCl for the top-most curve to 7.0 M GdHCl for the bottom-most curve). The insert on the right shows the shift of the maximum fluorescence emission wavelength as a function of denaturant concentration.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 80| Part 11| November 2024|
https://doi.org/10.1107/S2059798324009707
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