Figure 4
Oligomerization analysis of ΔP1 using SEC-MALS and a three-start helical model. MALS analysis of (a) unoligomerized and (b) oligomerized ΔP1 samples after performing inline SEC at a flow rate of 0.5 ml min−1. Images were created using the ASTRA software suite version 6.0 (Wyatt). (c) Overlaid UV280 nm chromatograms from both experimental runs. (d) A model for the assembly direction of the pilus using the ΔP1 pilin structure based on a three-start helical assembly (Petrov et al., 2013; Craig et al., 2006). Monomers are shown in green and cyan, with clashing residues in red and orange; the direction of pilin oligomerization would be from the cyan molecule to the green molecule. |