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Figure 6
Subtomogram averaging of rubisco molecules. A slice through a tomogram of rubisco-containing carboxysomes with centroids identified by the tomoCPT base model (a) and fine-tuned on a subset of data (b). The contours encircling the rubisco molecules are coloured by the confidence score attributed to that centroid by tomoCPT, with cool colours representing low confidence and warmer colours representing high confidence. (a) Fit of PDB entry 7zc1 into the Coulomb potential map derived from coordinates identified by the model fine-tuned on rubisco. (b) FSC trace of the map shown in (c). |