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![[Figure 1]](ag5052fig1mag.jpg)
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Figure 1
Crystallization and modeling of hDys-C10S-C188S-ABD1. (a) SDS–PAGE gel stained with Coomassie staining showing representative recombinant hDys-C10S-C188S-ABD1 used for crystallization (magenta arrow, 3 µg of protein loaded, molecular weight 28.6 kDa). (b) Size-exclusion chromatography of hDys-C10S-C188S-ABD1. The magenta brackets indicate the fractions pooled to obtain the material in (a). (c) Representative crystal of hDys-C10S-C188S-ABD1. (d) Molecular replacement with the AlphaFold2 model of hDys-C10S-C188S-ABD1 residues 2–246 showing the backbone (lines) and electron density (2Fo − Fc map contoured at 1.5σ). (e) Asymmetric unit of the final refined model: chains A, B, C and D are shown in green, cyan, magenta and yellow, respectively. Crystallographic waters are shown as red dots. |
![[Figure 1]](ag5052fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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