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Figure 3
SPR sensorgrams demonstrating the absence of self-interaction of h-5E5 Fab (a) and h-5E5 mAb (b). Fabs [h-5E5 (left) and 1104 control (right) in (a)] were amine-coupled at a high density (∼10 000 RUs) and h-5E5 Fab flowed aup to 24 µM. MAbs [h-5E5 (left) and 324 control (right) in (b)] were amine-coupled at a high density (∼25 000 RUs) and h-5E5 mAb flowed up to 10 µM. |