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![[Figure 24]](gm5113fig24mag.jpg)
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Figure 24
Unexpected ligands in autotaxin. (a) Domain organization in autotaxin. The two SMB domains are shown on the right in black and dark gray, the central PDE domain with the active-site Zn2+ in white and the inactive nuclease domain in brown to the left. (b) An additional 4-chlorotosyl group (red in the formula) is present in the hydantoin inhibitor (7g34), as detected both by an omit map contoured at 2.5 r.m.s.d. and as an impurity by mass spectrometry. (c) In 7g56, an inhibitor containing an acetylene group binds at the same site as the hydantoin inhibitor. (d) The acetylene inhibitor is dimerized by oxidation, i.e. it now has two triple bonds connected by a single bond. The duplicated part is shown in red in the formula. The dimer accounts for ∼90% of the total ligand and thus is the main product of the synthesis. |
![[Figure 24]](gm5113fig24.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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