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![[Figure 9]](gm5115fig9mag.jpg)
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Figure 9
Association of hFABP4 with micelles and bicelles. (a) Chemical structures of the lipids and detergent used. (b) Schematic view of the increased hFABP4 tumbling times in the presence of micelles and bicelles. The larger the assembly, the larger the tumbling times and the smaller the peak resolution and peak heights. (c) A negatively charged 20:80 mixture of long-chain DMPG:DMPC lipids was titrated with the short-chain detergent DHCP to arrive at the q-values indicated. The HSQC spectra are contoured at the same height for comparison. The spectrum on the left is the spectrum in aqueous solution as a reference. The spectra in the middle indicate binding of hFABP4 to charged micelles (three middle spectra) with increasing bicelle character as a function of q. No binding is observed to neutral DMPC/DHPC bicelles (right-hand spectrum). |
![[Figure 9]](gm5115fig9.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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