Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE

Van, C.T.S.; Reboul, C.F.; Caesar, J.J.E.; Meana-Pañeda, R.; Lountos, G.T.; Deme, J.C.; Bryant, O.J.; Johnson, S.; Piczak, C.T.; Valkov, E.; Lea, S.M.; Elmlund, H.

doi:10.1107/S2059798325005686

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 3
Impact of nsample on volume quality and compute time for the FliPQRFlhB complex. (a) Representative ab initio volumes calculated in SIMPLE using nsample values between 5000 and 25 000 with the computational cost relative to the time taken to calculate the 25 000 nsample volume below. (b) Ab initio volume obtained from the same particle set in cryoSPARC.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 81| Part 8| August 2025| Pages 396-409

https://doi.org/10.1107/S2059798325005686

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