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![[Figure 4]](ic5124fig4mag.jpg)
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Figure 4
Finding useable particles in streamed data sets using SIMPLE ab initio. For each sample, SIMPLE ab initio was run directly on all particles from the stream without any manual or automatic 2D class rejections. The percentages report the distribution of particles between the different states. Three states were arbitrarily chosen for (a) TRPM4, (c) FliPQRFLhB and (d) RNA polymerase, whereas in (e) the ribosome particles were arbitrarily divided into four states. (b) shows a TRPM4 ab initio 3D reconstruction obtained from a subset of particles from the initial junk partition of 65%, identified by subjecting this partition to further 2D analysis, selection of 2D classes and single-state ab initio 3D reconstruction. This TRPM4 conformation is likely to correspond to a previously identified calcium-bound cold state (Hu et al., 2024  ). |
![[Figure 4]](ic5124fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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