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![[Figure 2]](zha5001fig2mag.jpg)
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Figure 2
De novo identification of proteins with SPA. (a) Brief summary of the sample-preparation strategy applied to prepare bovine sperm doublet microtubules for SPA data collection. (b) Two orthogonal views of the 48 nm repeat of axonemal doublet microtubules from bovine sperm, highlighting all the microtubule inner proteins identified (PDB entry 8otz). (c) Two orthogonal views of the 96 nm repeat of axonemal doublet microtubules showing all the external complexes (PDB entry 9fqr). (d) Representative densities of three different microtubule inner proteins (Tektin-1, Tektin-2 and Tektin-4) and their corresponding sequences, illustrating how the protein sequence can be `read' from the density. |
![[Figure 2]](zha5001fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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