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![[Figure 2]](xh5060fig2mag.jpg)
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Figure 2
Effect of electron dose on the strength of integrated and unmerged mean reflection intensities 〈I〉. The flux density is approximately 0.10 e− Å−2 s−1 (dose rate 0.45 MGy s−1 at 200 keV and 0.38 MGy s−1at 300 keV) and the exposure time is 1.5 s per frame. (a) Loss of diffraction-spot strength/intensity and resolution due to radiation damage with increasing frame number. Panels (I) and (II) show the trend of intensity decay in lysozyme:native and HCA II:AZM MicroED data, respectively, collected on a Timepix detector installed on a Jeol JEM-2100 microscope operated at 200 kV. Panel (III) shows diffraction patterns of lysozyme:GdCl3 collected on a OneView Themis Z microscope operated at 300 kV. (b) Intensity of diffraction spots as a function of resolution for the four data sets. 1-10F, first ten frames; 1-20F, first 20 frames; 21-35F, frame range 21–35; All F, all data. Overall, the 1-10F data set yielded reflections with higher mean intensities compared with other data sets. Data are plotted as 〈I〉 = mean Ihkl ± standard error of the mean versus resolution for a representative 24 crystals (lysozyme:native), 16 crystals (HCA II:AZM) and nine crystals (lysozyme:GdCl3). Note that in each case the same crystals are compared for the different doses. Lysozyme:native refers to native tetragonal lysozyme crystals deposited on a holey carbon grid with nylon support, HCA II:AZM refers to HCA II bound to its inhibitor acetazolamide and lastly lysozyme:GdCl3 refers to lysozyme soaked in GdCl3. |
![[Figure 2]](xh5060fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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