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Figure 4
Site-specific damage of disulfide bonds in lysozyme:native as a function of electron dose. (a) Positions of the four disulfide bonds in hen egg-white lysozyme. (b) The 2mFo − DFc charge-density maps are shown as blue meshes with a contour level of 1.2σ above the mean. mFo − DFc difference densities are shown in green and red for positive and negative density, respectively, contoured at 2.5σ above and below the mean. A section covering 2 Å around atoms is shown for all densities. (PyMOL, Schrödinger). The electron doses are as specified in the figure. |
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