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![[Figure 2]](rr5256fig2mag.jpg)
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Figure 2
Representative two-dimensional template-matching (2DTM) results for searches run using a large ribosomal subunit (LSU), small ribosomal subunit body (SSU; modified from PDB entry 6q8y; Tesina et al., 2019  ) and 20S proteasome (PDB entry 1ryp; Groll et al., 1997) as reference templates. (a) Micrograph used for both LSU and SSU body 2DTM searches collected at 0.936 Å per pixel. (b) Left: LSU template with simulated molecular mass below. Right: 2DTM z-scores for LSU in the region of interest [red box in (a)]. (c) Left: SSU template with simulated molecular mass below. Right: 2DTM z-scores for SSU in the region of interest [red box in (a)]. (d, e) Enlarged z-score surface maps, output as scaled_mip from Leopard-EM within a cyan box for the LSU and SSU, respectively. (f, g, h) The relative position and orientations of LSU and SSU templates overlaid on the micrograph region of interest, where (f) shows the LSU alone, (g) shows both the LSU and SSU, and (h) shows the SSU alone. (i) Micrograph used for the 20S proteasome 2DTM search collected at 1.06 Å per pixel. (j) Left: proteasome template with simulated molecular mass below. Right: 2DTM z-scores for the proteasome search in the region of interest [red box in (i)]. (k) Z-score surface map for the identified proteasome peak. (l) 20S proteasome template at identified location and orientation overlaid on the region of interest. The scale bars in (f), (g), (h) and (l) correspond to 20 nm. |
![[Figure 2]](rr5256fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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